Light and Electron Microscopic Details of blood-Spleen Barrier in Nandanam Chicken (Gallus domesticus)

Light and electron microscopic studies of blood-spleen barrier was done in various age groups of Nandanam Chicken ranging from day-old to forty weeks. The spleen is the largest secondary lymphoid organ which can perform filtration of blood and also the primary site for imunocyte proliferation and differentiation. The results showed that the parenchyma was madeup of white and red pulp. The white pulp appeared as islands enclosed by red pulp and there was no distinct marginal zone between the red and white pulp. The trabeculae were not evident in all the age groups studied. The arterioles from the periphery of the white pulp observed to enter the red pulp as sheathed capillaries. These capillaries were found to be surrounded by reticular cells and macrophages which formed the ellipsoids or Sheathed capillaries. Under transmission electron microscope, the ellipsoids were found to have a meshwork of stellate reticular cells, reticular fibres and a few macrophages.